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The distance between Guadalajara Jalisco and Guanajuato Guanajuato is 170 miles or 273 kilometers.
It is possible to drive from Guadalajara to Guanajuato through San Pedro Tlaquepaque, Tonalá, León, Tepatitlán de Morelos, Pegueros, Valle de Guadalupe and San Juan de los Lagos.
You can calculate on how to get from Guadalajara to Guanajuato via transit cities by typing the city names in the "intermediate points" field in the "advanced options".